SEMESTER-I
PAPER II-NUTRITIONAL BIOCHEMISTRY
Total-40 hrs
S. No. | TOPICS TO BE COVERED | DOMAIN | TEACHING HOURS |
Module 1 |
|
Must Know Membrane structure and transport process across it. Desirable to Know |
2 |
Module2 |
|
Must Know |
2 |
Module 3 |
|
Must Know Carbohydrate and its transport, cellular metabolism pathways, regulation. Desirable to Know Disorder of carbohydratemetabolism. Nice to Know Classification & structural properties. |
6 |
Module4 |
|
Must Know Lipids and its transport, cellular metabolism pathways, regulation. Desirable to Know Metabolism of lipoproteins&disorders of lipid metabolism. Nice to Know Classification & structural properties. |
6 |
Module6 |
|
Must Know Protein and its transport, cellular metabolism pathways, regulation. Desirable to Know |
8 |
Module7 |
|
Must Know Biological Oxidation, Oxidative phosphorylation. Tricarboxylic acid cycle, Electron transport chain. Desirable to Know Starve-feed cycle. Caloric homeostasis & futile cycles. |
4 |
Module8 |
|
Must Know |
4 |
Module9 |
|
Must Know Biochemistry of DNA&RNAs replication,Mutation, repair & recombination. Desirable to Know Disorders of nucleic acid metabolism. |
4 |
Module10 |
|
Must Know Enzyme, its synthesis and regulation, detoxification in the bodyand enzymes in clinical diagnosis. Desirable to Know Metabolism of xenobiotics, free radicals, ROS & oxidative damage |
4 |
Practical Must Know Total-20 hrs
- Determination of pH(in acids, alkalis and buffers using pH meter and indicators).
- Colorimeter-calibration graph
- Separation Technique-Chromatography(paper and column),Centrifugation and Electrophoresis
- Estimation of Hb by Cyanmethoemoglobin method or Sahl’s method.
- Estimation of fat (centrifugation or soxhlet method)
- Estimation of proteins(by kjeldahl method)
- Estimation of fibre.
- Estimation of ash
- Estimation of moisture
- Estimation of ascorbic acid(titrimetric/ colorimetric method/)
- Estimation of calcium (titrimetric method/)
- Estimation of iron(wong’s method)
- Lipid profile in given blood samples
- Study the principle and working of Glucometer
References:
- Murray, R.K., Granner, D.K., Mayes, P.A. and Rodwell, V.W. (2000): 25th Ed. Harpers Biochemistry. Macmillan Worth Publishers.
- Nelson, D.L. and Cox, M.M. (2000): 3rd Ed. Lehninger’s Principles of Biochemistry, Macmillan Worth Publishers.
- Devlin, T.M. (1997): 4th Ed. Text book of Biochemistry with Clinical Correlations, Wiley Liss Inc
- Stryer, L. (1998): 4th Ed. Biochemistry, WH Freeman and Co.
- Conn, E.E., Stumpf, P.K., Bruening, G. and Doi, R.H. (2001): 5th Ed. Outlines of Biochemistry, John Wiley and Sons.
- Voet, D. Voet, J.G. and Pratt, C.W. (1999). Fundamentals of Biochemistry.
- Tietz, N.W. (1976) Fundamentals of Clinical Chemistry. WB Saunders Co.
- King, E.J. and Wootton, I.D.P. (1956). 3rd ed. Micro-Analysis in Medical Biochemistry. J and A Churchill Ltd.
- Plummer, D.T. (1987). 3rd ed. An Introduction to Practical Biochemistry. McGraw-Hill Book Co.