Syllabus | PAPER II-NUTRITIONAL BIOCHEMISTRY | M.Sc. Nutrition & Dietetics

SEMESTER-I
PAPER II-NUTRITIONAL BIOCHEMISTRY

Total-40 hrs

S. No.	TOPICS TO BE COVERED	DOMAIN	TEACHING HOURS
Module 1	Introduction to membrane structures Composition of membrane structures Transport process across cell membranes	Must Know Membrane structure and transport process across it. Desirable to Know Composition of membrane structures.	2
Module2	Introduction ,Acid, Base & pH Acid base balance and its regulationin human body	Must Know	2
Module 3	Introduction to Carbohydrate Intestinal transport of carbohydrates. Transport of glucose across various cells. Cellular metabolism of carbohydrates. Glycogen metabolism Regulation of carbohydrate metabolism at substrate level, enzyme level, hormonal level and organ level. Disorders of carbohydrate metabolism. Definition, classification, structure and properties of glycoprotein & Proteoglycans.	Must Know Carbohydrate and its transport, cellular metabolism pathways, regulation. Desirable to Know Disorder of carbohydratemetabolism. Nice to Know Classification & structural properties.	6
Module4	Intestinal transport of lipids. Cellular uptake & metabolism of lipids (beta-oxidation, synthesis and breakdown of unsaturated fatty acids, cholesterol, phospholipids & triacylglycerol) Lipoprotein metabolism, VLDL and LDL (‘Forward’ Cholesterol transport) VLDL and LDL (Endogenous TAG transport), HDL (‘Reverse’ Cholesterol transport) Regulation of lipid metabolism at substrate level, enzyme level, hormonal level and organ level. Disorders of lipid metabolism, Dyslipidemia, Lipid storage diseases.	Must Know Lipids and its transport, cellular metabolism pathways, regulation. Desirable to Know Metabolism of lipoproteins&disorders of lipid metabolism. Nice to Know Classification & structural properties.	6
Module6	Metabolism of amino acids. Biosynthesis & catabolism glucose and ketone bodies, protein, amino acids, non-protein amino acids (urea cycle, transamination, one-carbon metabolism) Creatine and creatinine Plasma proteins – Nature, properties and functions. Biologically active peptides, polypeptides and transport proteins Inborn errors of amino acid metabolism.	Must Know Protein and its transport, cellular metabolism pathways, regulation. Desirable to Know Inborn error of protein metabolism. Nice to Know Classification & structural properties.	8
Module7	Introduction to intermediary metabolism Starve-feed cycle. Caloric homeostasis & futile cycles. Tricarboxylic acid cycle Biological Oxidation Electron transport chain Oxidative phosphorylation.	Must Know Biological Oxidation, Oxidative phosphorylation. Tricarboxylic acid cycle, Electron transport chain. Desirable to Know Starve-feed cycle. Caloric homeostasis & futile cycles.	4
Module8	Biochemical aspects of purine and pyrimidines Role of purine & pyrimidine nucleotides in metabolism	Must Know	4
Module9	Biochemistry of DNA&RNAs DNA replication,Mutation, repair & recombination Disorders of nucleic acid metabolism	Must Know Biochemistry of DNA&RNAs replication,Mutation, repair & recombination. Desirable to Know Disorders of nucleic acid metabolism.	4
Module10	Introduction to enzyme & its synthesis Kinetics of mono-substrate and bi-substrate catalyzed reactions Enzyme specificity Regulation of enzyme activity Enzymes in clinical diagnosis Enzyme detoxification in the body Metabolism of xenobiotics, free radicals, ROS & oxidative damage	Must Know Enzyme, its synthesis and regulation, detoxification in the bodyand enzymes in clinical diagnosis. Desirable to Know Metabolism of xenobiotics, free radicals, ROS & oxidative damage	4

Practical Must Know Total-20 hrs

Determination of pH(in acids, alkalis and buffers using pH meter and indicators).
Colorimeter-calibration graph
Separation Technique-Chromatography(paper and column),Centrifugation and Electrophoresis
Estimation of Hb by Cyanmethoemoglobin method or Sahl’s method.
Estimation of fat (centrifugation or soxhlet method)
Estimation of proteins(by kjeldahl method)
Estimation of fibre.
Estimation of ash
Estimation of moisture
Estimation of ascorbic acid(titrimetric/ colorimetric method/)
Estimation of calcium (titrimetric method/)
Estimation of iron(wong’s method)
Lipid profile in given blood samples
Study the principle and working of Glucometer

References:

Murray, R.K., Granner, D.K., Mayes, P.A. and Rodwell, V.W. (2000): 25th Ed. Harpers Biochemistry. Macmillan Worth Publishers.

Nelson, D.L. and Cox, M.M. (2000): 3rd Ed. Lehninger’s Principles of Biochemistry, Macmillan Worth Publishers.

Devlin, T.M. (1997): 4th Ed. Text book of Biochemistry with Clinical Correlations, Wiley Liss Inc

Stryer, L. (1998): 4th Ed. Biochemistry, WH Freeman and Co.

Conn, E.E., Stumpf, P.K., Bruening, G. and Doi, R.H. (2001): 5th Ed. Outlines of Biochemistry, John Wiley and Sons.
Voet, D. Voet, J.G. and Pratt, C.W. (1999). Fundamentals of Biochemistry.
Tietz, N.W. (1976) Fundamentals of Clinical Chemistry. WB Saunders Co.

King, E.J. and Wootton, I.D.P. (1956). 3rd ed. Micro-Analysis in Medical Biochemistry. J and A Churchill Ltd.

Plummer, D.T. (1987). 3rd ed. An Introduction to Practical Biochemistry. McGraw-Hill Book Co.

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Syllabus | PAPER II-NUTRITIONAL BIOCHEMISTRY | M.Sc. Nutrition & Dietetics | First Semester