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Syllabus | PAPER II-NUTRITIONAL BIOCHEMISTRY | M.Sc. Nutrition & Dietetics | First Semester

SEMESTER-I
PAPER II-NUTRITIONAL BIOCHEMISTRY  

Total-40 hrs

S. No. TOPICS TO BE COVERED DOMAIN TEACHING HOURS
Module 1
  • Introduction to membrane structures
  • Composition of membrane structures
  • Transport process across cell membranes
 Must Know
Membrane structure and transport process across it.

Desirable to Know
Composition of membrane structures.

2
Module2  

  • Introduction ,Acid, Base & pH
  • Acid base balance and its regulationin human body
  

Must Know

2
 

Module 3
  • Introduction to Carbohydrate
  • Intestinal transport of carbohydrates.
  • Transport of glucose across various cells.
  • Cellular metabolism of carbohydrates.
  • Glycogen metabolism
  • Regulation of carbohydrate metabolism at substrate level, enzyme level, hormonal level and organ level.
  • Disorders of carbohydrate metabolism.
  • Definition, classification, structure and properties of glycoprotein &  Proteoglycans.
 Must Know
Carbohydrate and its transport, cellular metabolism pathways, regulation.
Desirable to Know
Disorder of carbohydratemetabolism.
Nice to Know
Classification & structural properties.

 

6
Module4  

  • Intestinal transport of lipids.
  •  Cellular uptake & metabolism of lipids (beta-oxidation, synthesis and breakdown of unsaturated fatty acids, cholesterol, phospholipids & triacylglycerol)
  • Lipoprotein metabolism, VLDL and LDL (‘Forward’ Cholesterol transport) VLDL and LDL (Endogenous TAG transport), HDL (‘Reverse’ Cholesterol transport)
  •  Regulation of lipid metabolism at substrate level, enzyme level, hormonal level and organ level.
  • Disorders of lipid metabolism, Dyslipidemia, Lipid storage diseases.
 Must Know
Lipids and its transport, cellular metabolism pathways, regulation.
Desirable to Know
Metabolism of lipoproteins&disorders of lipid metabolism.
Nice to Know
Classification & structural properties.

 

6
Module6
  • Metabolism of amino acids.
  • Biosynthesis & catabolism glucose and ketone bodies, protein, amino acids, non-protein amino acids (urea cycle, transamination, one-carbon metabolism)
  • Creatine and creatinine
  • Plasma proteins – Nature, properties and functions.
  • Biologically active peptides, polypeptides and transport proteins
  • Inborn errors of amino acid metabolism.
 Must Know
Protein and its transport, cellular metabolism pathways, regulation.

Desirable to Know
Inborn error of protein metabolism.
Nice to Know
Classification & structural properties.

 

 

8
Module7
  • Introduction to intermediary metabolism
  • Starve-feed cycle.
  • Caloric homeostasis & futile cycles.
  • Tricarboxylic acid cycle
  • Biological Oxidation
  • Electron transport chain
  • Oxidative  phosphorylation.
 Must Know
Biological Oxidation, Oxidative phosphorylation.
Tricarboxylic acid cycle, Electron transport chain.
Desirable to Know
Starve-feed cycle.
Caloric homeostasis & futile cycles.

 

4
Module8
  • Biochemical aspects of purine and pyrimidines
  • Role of purine & pyrimidine nucleotides in metabolism
 Must Know

 

4
Module9
  • Biochemistry of DNA&RNAs
  • DNA replication,Mutation, repair &  recombination
  • Disorders of nucleic acid metabolism
 Must Know
Biochemistry of DNA&RNAs replication,Mutation, repair & recombination.
Desirable to Know
Disorders of nucleic acid metabolism.

 

4
Module10
  • Introduction to enzyme  & its synthesis
  • Kinetics of mono-substrate and bi-substrate catalyzed reactions Enzyme specificity
  • Regulation of enzyme activity
  • Enzymes in clinical diagnosis
  • Enzyme detoxification in the body
  • Metabolism of  xenobiotics, free radicals, ROS & oxidative damage
 Must Know
Enzyme, its synthesis and regulation, detoxification in the bodyand enzymes in clinical diagnosis.
Desirable to Know
Metabolism of  xenobiotics, free radicals, ROS & oxidative damage

 

4

Practical       Must Know  Total-20 hrs

  • Determination of pH(in acids, alkalis and buffers using pH meter and indicators).
  • Colorimeter-calibration graph
  • Separation Technique-Chromatography(paper and column),Centrifugation and Electrophoresis
  • Estimation of Hb by Cyanmethoemoglobin method or Sahl’s method.
  • Estimation of fat (centrifugation or soxhlet method)
  • Estimation of proteins(by kjeldahl method)
  • Estimation of fibre.
  • Estimation of ash
  • Estimation of moisture
  • Estimation of ascorbic acid(titrimetric/ colorimetric method/)
  • Estimation of calcium (titrimetric method/)
  • Estimation of iron(wong’s method)
  • Lipid profile in given blood samples
  • Study the principle and working of Glucometer

References:

  • Murray, R.K., Granner, D.K., Mayes, P.A. and Rodwell, V.W. (2000): 25th Ed. Harpers Biochemistry. Macmillan Worth Publishers.
  • Nelson, D.L. and Cox, M.M. (2000): 3rd Ed. Lehninger’s Principles of Biochemistry, Macmillan Worth Publishers.
  • Devlin, T.M. (1997): 4th Ed. Text book of Biochemistry with Clinical Correlations, Wiley Liss Inc
  • Stryer, L. (1998): 4th Ed. Biochemistry, WH Freeman and Co.
  • Conn, E.E., Stumpf, P.K., Bruening, G. and Doi, R.H. (2001): 5th Ed. Outlines of Biochemistry, John Wiley and Sons.
  • Voet, D. Voet, J.G. and Pratt, C.W. (1999). Fundamentals of Biochemistry.
  • Tietz, N.W. (1976) Fundamentals of Clinical Chemistry. WB Saunders Co.
  • King, E.J. and Wootton, I.D.P. (1956). 3rd ed. Micro-Analysis in Medical Biochemistry. J and A Churchill Ltd.
  • Plummer, D.T. (1987). 3rd ed. An Introduction to Practical Biochemistry. McGraw-Hill Book Co.
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