Syllabus | Mycology, Phycology and Virology | First Year | B.Sc Microbiology

Subject: Mycology, Phycology and Virology

S.no

Topic

Domain

Hours

1

Classification and application of fungi: General classification and

Life cycle, structure and occurrence – (i) Cellular slime molds ( ii) True slime mold  (iii) Oomycetes (iv) Chytridiomycetes (v) Zygomycetes (vi) Ascomycetes (vii)Basidiomycetes  (viii) Deuteromycetes

Must know

12

Bioremediation (of wood, paper, textile, leather), mycotoxins

Desirable to know

6

Economic importance of fungi with examples in agriculture, environment, industry, medicine, food,

Nice to know

2

2

Classification and application of algae: General classification

Life cycle, thallus organisation and occurrence – (i) Chlorophyceae ( ii) Charophyceae (iii) Diatoms (iv) Xanthophyceae (v) Phaeophyceae (vi) Rhodophyceae: (vii) Cyanobacteria

Must know

14

Lichens

Desirable to know

2

Economic importance of algae with examples in agriculture, environment, industry and food.

Nice to know

4

3

Introduction and viral taxonomy: History of viruses, definition of viruses, general properties of viruses, viroids, virusoids, satellite viruses and prions
Structure and salient features of viral genomes: Capsid symmetry, enveloped and non-enveloped viruses. TMV, T4 phage, Hepatitis B virus, Picornavirus, Rhabdovirus, Retrovirus, Influenza virus.

Must know

13

Classification and nomenclature of viruses. Applications of virology.

Desirable to know

5

Isolation, purification, cultivation of viruses,

Nice to know

2

4

Bacteriophages: Definition, structure and cycle of T4 and lambda phage,

 Types of oncogenic DNA and RNA viruses. Concepts of oncogenes, proto oncogenes, tumor suppressor genes.

Must know

12

Transmission, prevention and control of viral diseases: Persistent and non-persistent mode. Antiviral compounds, interferons and viral vaccines.

Desirable to know

4

Viral multiplication

Nice to know

4

5

PRACTICAL

Study of the vegetative and reproductive structures of following genera through temporary and permanent slides: Mucor, Saccharomyces, Penicillium, Agaricus and Alternaria

Study of the following genera through temporary and permanent slides: Volvox, and Nostoc

To study structure of important animal viruses (rhabdo, influenza, paramyxo, Hepatitis B & retroviruses) using electron micrographs/photographs

Must know

25

Preparation of Potato Dextrose Medium

To study structure of important plant viruses (caulimo, gemini, tobacco ring spot, cucumber mosaic & alpha-alpha mosaic viruses) using electron micrographs/photographs

Desirable to know

10

Study of cytopathic effects using photographs

Study of the following genera through temporary and permanent slides: Coleochaete, Vaucheria, Ectocarpus, Polysiphonia

Nice to know

5

Suggested readings:

  1. Kumar HD. (1990). Introductory Phycology. 2nd edition. Affiliated East Western Press.
  2.  Kumar HD. (1995). The Text Book on Algae. 4th edition. Affiliated East Western Press
  3. Alexopoulos CJ, Mims CW and Blackwell M. (1996). Introductory Mycology. 4th edition. John Wiley and Sons, Inc
  4. Flint SJ, Enquist, LW, Krug, RM, Racaniello, VR, Skalka, AM (2004). Principles of Virology, Molecular biology, Pathogenesis and Control. 2nd edition. ASM press
    Washington DC.
  5. Levy JA, Conrat HF, Owens RA. (2000). Virology. 3rd edition. Prentice Hall publication, New Jersey
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