SYLLABUS | DIPLOMA (MLT) | MICROBIOLOGY | SECOND YEAR

Morphology

Classification of fungi

Cultivation of fungi

stains used in mycology

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Desirable to know

Nice to know

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Superficial Mycoses:

1. Dermatophytes,
2. Candidiasis.

1. Mycetoma,
2. Rhinosporidium, and Sporotrichosis

1. Histoplasmosis, Blastomycosis
2. Cryptococcosis

• Aspergillosis, Pencillosis,
• Zygomycosis,
• Pneumocystis

Must know

Nice to know

Must know

1.      Classification

and morphology of Protozoa

Classification and morphology and Helminthes

Nice to know

 Must know

 Nice to know

i.            Protozoa :

a)      Entamoeba,

b)      Trichomonas and Giardia,

c)      Plasmodium,

d)      Lieshmania

e)      Toxoplasma,

f)       Cryptosporidium & other coccidian parasites.

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Must know

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a)         Taenia,

b)         Echinococcus.

c)         Ascaris,

d)         Ancylostoma, Strongyloides,

e)         Trichuris, &Enterobius, Filaria

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1. General properties of Viruses
2. Collection, transportation and storage of samples for viral diagnosis.
3. Cultivation of viruses.
4. Bacteriophages

  Must know

 Desirable to know

 Nice to know

Disease caused (pathogenesis), lab diagnosis, and prevention of:

1. Herpes viruses ,
2. Viral Hepatitis,
3. Human Immunodeficiency Viruses ,
4. Rabies,
5. Poliomyelitis,
6. Influenza Viruses
7. Rubella, Mumps , Measles
8. Rota virus.
9. Japanese encephalitis & Dengue,
10. Chikungunya,
11. Kyasanur Forest disease
12. Human Onocogenic Viruses.

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Specimen processing

• Blood
• Sputum, throat swab, nasopharyngeal swab, Swabs (pus, wound )
• CSF and other body fluids, Stool and rectal swabs.

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Hospital infection control

Health care associated infection

Emerging infectious diseases

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(Total-30)

i)       Demonstration of fungi using KOH , Lactophenol cotton blue and India ink

ii)     Colony characteristics and Microscopic examination and identification tests for :

iii)   Candida and  Cryptococcus,

iv)   Dermatophytes

v)       Aspergillus

Parasitology:

I.            Stool examination: Saline mount, Iodine mount

II.            Stool concentration techniques

III.            Preparation of thick and thin smears

1. Spottests/ELISA: HBV, HCV,HIV, Dengue X2

Must know

vii) Slide culture technique

I.            Preparation and staining technique of Leishman’s stain and Giemsa stain

II.            Demonstration of malarial parasite in peripheral smear

Rapid test for malaria and QBC

Desirable to know

1. Demonstration of embryonatedegginoculationDemonstration of cell culture techniques and Cytopathic effect

Demonstration of heamagglutination and heamagglutination inhibition assay

Nice to know