SYLLABUS | DIPLOMA (MLT) | MICROBIOLOGY | SECOND YEAR

Syllabus (Microbiology paper II)

 

Sl. No Topics Domain Time (Total-70)
1

 

Mycology

Morphology

Classification of fungi

Cultivation of fungi

stains used in mycology

 

Must know

Desirable to know

Nice to know

Must know

 

1

1

 

1

1

Lab diagnosis of fungal Infections

Superficial Mycoses:

    1. Dermatophytes,
    2. Candidiasis.

 

Must know 3
Subcutaneous Mycoses:

    1. Mycetoma,
    2. Rhinosporidium, and Sporotrichosis
Desirable to know 3
Systemic Mycoses:

    1. Histoplasmosis, Blastomycosis
    2. Cryptococcosis
Must know 3
Opportunistic Fungi:

  • Aspergillosis, Pencillosis,
  • Zygomycosis,
  • Pneumocystis
 

Must know

Nice to know

Must know

3
2 Parasitology

1.      Classification

and morphology of Protozoa

 

Classification and morphology and Helminthes

 

Nice to know

 Must know

 Nice to know

4
1.      Structure, life cycle pathogenesis &  laboratory diagnosis of following parasites

i.            Protozoa :

a)      Entamoeba,

b)      Trichomonas and Giardia,

c)      Plasmodium,

d)      Lieshmania

e)      Toxoplasma,

f)       Cryptosporidium & other coccidian parasites.

 Must know

 

 

Nice to know

 

 

Must know

 

4

 

  

2

  

 

2

               ii.            Helminthes :

a)         Taenia,

b)         Echinococcus.

c)         Ascaris,

d)         Ancylostoma, Strongyloides,

e)         Trichuris, &Enterobius, Filaria

Must know

  

Nice to know

 

Must know

3

 

2

 

2

3

 

Virology

  1. General properties of Viruses
  2. Collection, transportation and storage of samples for viral diagnosis.
  3. Cultivation of viruses.
  4. Bacteriophages
 

  Must know

 Desirable to know

 Nice to know

5
 

Disease caused (pathogenesis), lab diagnosis, and prevention of:

  1. Herpes viruses ,
  2. Viral Hepatitis,
  3. Human Immunodeficiency Viruses ,
  4. Rabies,
  5. Poliomyelitis,
  6. Influenza Viruses
  7. Rubella, Mumps , Measles
  8. Rota virus.
  9. Japanese encephalitis & Dengue,
  10. Chikungunya,
  11. Kyasanur Forest disease
  12. Human Onocogenic Viruses.
 

 

 

Must know

 

Nice to know

 

Desirable to know

  

8

 

 

1

 

 

3

4  

Specimen processing

  • Blood
  • Sputum, throat swab, nasopharyngeal swab, Swabs (pus, wound )
  • CSF and other body fluids, Stool and rectal swabs.
 

Must know

Desirable to know

Must know

 3

1

1

5 Applied Microbiology

Hospital infection control

Health care associated infection

Emerging infectious diseases

 

Must know

Must know

Nice to know

 

2

4

2

 

Syllabus (Microbiology practical paper II)

 

S. No Topic Domain Hours

(Total-30)

1. Mycology::

i)       Demonstration of fungi using KOH , Lactophenol cotton blue and India ink

ii)     Colony characteristics and Microscopic examination and identification tests for :

iii)   Candida and  Cryptococcus,

iv)   Dermatophytes

v)       Aspergillus

Parasitology:

I.            Stool examination: Saline mount, Iodine mount

II.            Stool concentration techniques

III.            Preparation of thick and thin smears

  1. Spottests/ELISA: HBV, HCV,HIV, Dengue X2
 

 

 

 

Must know

20
2. vi)   Miscellaneous fungi

vii) Slide culture technique

I.            Preparation and staining technique of Leishman’s stain and Giemsa stain

II.            Demonstration of malarial parasite in peripheral smear

Rapid test for malaria and QBC

 

 

 

Desirable to know

5
3.
  1. Demonstration of embryonatedegginoculationDemonstration of cell culture techniques and Cytopathic effect

Demonstration of heamagglutination and heamagglutination inhibition assay

 

 

Nice to know

5
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