MICROBIOLOGY 1st Year
For Syllabus (Microbiology paper 1)
| Sl. No | Topics | Domain | Time
(Total-70 hrs) |
| 1 | General Microbiology
Microscopy Light microscope, Dark field, Phase contrast microscopy, Fluorescent & Electron microscopy |
Must know
Desirable to know Nice to know Must know |
1
1 1 1
|
| 2 | Sterilization and Disinfection
Physical Methods of Sterilization Chemical Methods of Sterilization Methods of Disinfection |
Must know |
2 |
| 3 | Growth,
Nutrition & Metabolism of Bacteria |
Must know
Desirable to know Nice to know |
1
1 1 |
| 4 | Bacterial genetics | Must know | 5 |
| 5 | Bacterial Culture and Identification
(a) Culture Media & Transport Media (b)Sample collection and transport Methods (c)Aerobic Bacterial Culture Techniques (d)Anaerobic Bacterial Culture Techniques (e)Smear preparation & Staining methods (f)Principle and techniques of biochemical Test |
Must know
Must know Must know Nice to know Must know Must know |
1
1
3
1
1 1 |
| 6 | Morphology,
Classification & infection caused by Microorganisms (in brief) Bacteria Morphology, Classification Human infection caused by bacteria |
Must know
Nice to know Must to know |
1
1
2 |
| 7 | Viruses
Morphology, Classification Human infection caused by Viruses |
Must know
Nice to know Must know |
1
1 2 |
| 8 | Fungi
Morphology, Classification Human infection caused by Fungi |
Must know
Nice to know Must know |
1
1 2 |
| 9 | Parasites
Morphology, Classification Human infection caused by Parasite |
Must know Nice to know Must know |
1
1
2 |
| 10 | Immunology
Immunity Antigen & Antibody Antigen antibody reactions I Precipitation, Agglutination Antigen antibody reactions II Complement fixation, Neutralization, ELISA, RIA, IF |
Must know Must know Desirable to know Nice to know |
2
5 5 3
|
| 11 | Antimicrobial Sensitivity Testing
-Antimicrobials and their mode of action -Mechanisms of drug resistance -Antimicrobial susceptibility testing (a)Diffusion Methods (b)Dilution Methods |
Must know
Must know Nice to know
Must know |
1
2 1
3 |
| 12 | Systemic Bacteriology
|
Must know
Desirable to know
Must know
Nice to know |
2
2
5
1 |
Syllabus (Microbiology practical paper 1)
| S. No | Topic | Domain | Hours
Total-30 |
| 1. | 1. Microscope
a. Light Microscope 2. Staining a. Gramsstaining b. ZN staining 3. Preparation of commonly used culture media a. Nutrient Agar b. Blood Agar c. Chocolate agar d. Mac Conkey agar e. Muller Hinton agar 4. Culturemethods a. Streak method b. Lawn method c. Stroke method d. Stab method e. Pour Plate method f. Liquid method 5. Identificationofbacterialculture a. Colonycharacteristics b. Morphological characteristics 6. Antibiotic susceptibility test a. Diffusion methods b. Dilution Methods 7. Bio medical waste a. Use of colour coded bags b. Black c. Blue d. Red e. Yellow 8. Demonstration of Sterilization & Disinfection method a. Autoclave b. Hot Air oven c. Chemical Sterilization 9. Collection of specimen a. From outpatient units b. Inpatient units c. Minor operation theatre d. Major operation theatre for sterility testing 10. Demonstration of personal protective equipment |
Must know
Must know
Must know
Must know
Must know
Must know
Must know
Must know
Must know
Must know |
2
2
2
2
2
2
2
2
2
2 |
| 2. | 1. Disinfection of wards, OT and Laboratory
2. Visit to CSSD |
Desirable to know | 5 |
| 3. | Demonstration of Sterilization & Disinfection method
a. Water bath b. Inspissator Sterility testing o Methods o Interpretation
|
Nice to know | 5 |
MICROBIOLOGY REFERENCES:
Laboratory acquired infections by C.H. Collins
Manual of Clinical Laboratory methods by Copal E Hopier
Medical Laboratory Methods ByBeuer
Bailey & Scott’s diagnostic microbiology (13th ed.) by Tille, P. M.
Elsevier’s medical laboratory science examination review by Graeter, L. J., Hertenstein, E. G., Accurso, C. E., &Labiner, G. H.
Fundamentals of urine and body fluid analysis (3rd ed.) by Brunzel, N. A.
Immunology and serology in laboratory medicine (5th ed.) by Turgeon, M. L.