SYLLABUS | DIPLOMA (MLT) | BIOCHEMISTRY | FIRST YEAR

BIOCHEMISTRY 1ST Year                    

COURSE SYLLABUS FOR THEORY:-

 

Sl.No TOPICS TO BE COVERED    Domain TEACHING HOURS
MODULE/ Chapter 1 Specimen Collection:

Types of Specimens

Method of specimen collection (Blood, serum, Urine and others)

Pre-analytical & analytical variables

Use of preservatives in specimen collection

Use of proper Anticoagulants in specimen collection

 Introduction to Laboratory Apparatus:

Overview of the functioning of Clinical Biochemistry.

Introduction to glass wares:

Pipettes and their Calibration (different types of pipettes like graduated, volumetric and automated pipettes).

Burettes and Beakers.

Flasks and their applications (volumetric, conical and round bottomed).

Reagent Bottles (graduated, common, Wash bottles and specimen bottles).

Funnels and their uses.

Measuring cylinders.

Test tubes and serum tubes.

Test tube draining rack, bottle racks, Pipette stands, tripod stand, wire gauze and Bunsen burner.

Cuvettes and their application in colorimetery and spectrdophotometry.

Bottle Dispensers and their Maintenance.

Maintenance, Care and cleaning of laboratory glassware.

Introduction to the Laboratory Instruments and their use, care & maintenance:

Water bath

Oven

Incubators

Water Distillation Plant and Deionizers

Refrigerators

Centrifuges

Laboratory Balance and Direct Readout Electrical Balances

Colorimeter

 

Spectrophotometer

pH Meter and its Calibration

 

Conventional and SI units used in the Laboratory

Molecular and equivalent weight

Normality, molality, molarity

Concentrations of solutions by w/w, w/v, v/v etc.

Preparation of standard solutions

Molar solutions and Percent solutions

 

Dilutions of Solutions or Samples:

Preparation of a stock standard and working standard.

Proper method of dilution of a solution or a laboratory sample.

Serial dilutions of samples

Saturated and supersaturated solutions

Significance of volumetric flask in preparing standard solutions,

 

Basic concept of Acids, Bases, Salts and Indicators

Acid, base, salts and buffers

Indicators and their Functions

Buffers of the body

 

 

Must Know

 

 

 

 

 

 

 

 

 

Must to know

 

 

 

 

 

 

 

 

 

 

 

MUST KNOW

Centrifuge, principle and application

Colorimeter principle, parts and            application

pH Meter principle, parts and application

 

 

 

 

 

Must to know

 

 

 

 

 

Nice to know

 

 

 

 

 

 

 

 

 

 

Nice to know

 

 

 

 

 

 

10

 

 

 

 

 

 

 

 

 

 

15

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

15

 

 

 

 

 

 

 

 

 

 

 

 

5

 

 

 

 

 

 

20

 

  

 

 

5

 

 SYLLABUS FOR BIOCHEMISTRY PRACTICAL:

 

Sl.No TOPICS TO BE COVERED Domain TEACHING HOURS
MODULE/ Chapter 1 pH Meter

Acid, base, salts and buffers

Demonstration of pH meter

General tests of biomolecules

General tests of carbohydrates

General test of proteins

General test of liquids

Urine analysis

Normal urine

Abnormal constituents of urine

Colorimetry

Princilples of colorimetry (Lambert and Beer’s laws and their verification)

Standard curve

 

Estimation of Blood sugar

Estimation of serum proteins.

 

 

 

 

 

 

 

 

Must Know

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

30

 

 

BIOCHEMISTRYREFERENCES:

Syllabus Books

Text Book of Biochemistry 4th edition – U. Satyanarayana and Chakrapani.

Text Book of Medical Laboratory Technology 5th edition – Praful B. Godkar.

Text Book of Medical Laboratory Technology 5th edition – RamnikSood.

Text Book of Biochemistry for Medical Students 5th edition – DM Vasudevan.

Text Book of Medical Biochemistry 3rd edition. Dinesh Puri.

Reference Books & Readings

Victor W. Rodwell, David A. Bender. Harper’s Illustrated Biochemistry, 30th edition. A Lange Medical book (2015).

Richard Harvey, Denish Ferrier. Lippincott’s Illustrated Reviews Biochemistry 5th edition.

District Laboratory Practice in Tropical Countries 3rd edition (vol. 1) – Monika Cheesbrough.

District Laboratory Practice in Tropical Countries 3rd edition (vol. 2) – Monika Cheesbrough.

Manual of Laboratory & Diagnostic Tests – Mcgraw Hill.

Drew Provan, Andrew Krentz. Oxford Handbook of Clinical and Laboratory Investigation 2nd edition. Oxford University Press.

SGTUNIVERSITY
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