BIOCHEMISTRY 1ST Year
COURSE SYLLABUS FOR THEORY:-
| Sl.No | TOPICS TO BE COVERED | Domain | TEACHING HOURS |
| MODULE/ Chapter 1 | Specimen Collection:
Types of Specimens Method of specimen collection (Blood, serum, Urine and others) Pre-analytical & analytical variables Use of preservatives in specimen collection Use of proper Anticoagulants in specimen collection Introduction to Laboratory Apparatus: Overview of the functioning of Clinical Biochemistry. Introduction to glass wares: Pipettes and their Calibration (different types of pipettes like graduated, volumetric and automated pipettes). Burettes and Beakers. Flasks and their applications (volumetric, conical and round bottomed). Reagent Bottles (graduated, common, Wash bottles and specimen bottles). Funnels and their uses. Measuring cylinders. Test tubes and serum tubes. Test tube draining rack, bottle racks, Pipette stands, tripod stand, wire gauze and Bunsen burner. Cuvettes and their application in colorimetery and spectrdophotometry. Bottle Dispensers and their Maintenance. Maintenance, Care and cleaning of laboratory glassware. Introduction to the Laboratory Instruments and their use, care & maintenance: Water bath Oven Incubators Water Distillation Plant and Deionizers Refrigerators Centrifuges Laboratory Balance and Direct Readout Electrical Balances Colorimeter
Spectrophotometer pH Meter and its Calibration
Conventional and SI units used in the Laboratory Molecular and equivalent weight Normality, molality, molarity Concentrations of solutions by w/w, w/v, v/v etc. Preparation of standard solutions Molar solutions and Percent solutions
Dilutions of Solutions or Samples: Preparation of a stock standard and working standard. Proper method of dilution of a solution or a laboratory sample. Serial dilutions of samples Saturated and supersaturated solutions Significance of volumetric flask in preparing standard solutions,
Basic concept of Acids, Bases, Salts and Indicators Acid, base, salts and buffers Indicators and their Functions Buffers of the body |
Must Know
Must to know
MUST KNOW Centrifuge, principle and application Colorimeter principle, parts and application pH Meter principle, parts and application
Must to know
Nice to know
Nice to know
|
10
15
15
5
20
5 |
SYLLABUS FOR BIOCHEMISTRY PRACTICAL:–
| Sl.No | TOPICS TO BE COVERED | Domain | TEACHING HOURS |
| MODULE/ Chapter 1 | pH Meter
Acid, base, salts and buffers Demonstration of pH meter General tests of biomolecules General tests of carbohydrates General test of proteins General test of liquids Urine analysis Normal urine Abnormal constituents of urine Colorimetry Princilples of colorimetry (Lambert and Beer’s laws and their verification) Standard curve
Estimation of Blood sugar Estimation of serum proteins. |
Must Know
|
30
|
BIOCHEMISTRYREFERENCES:
Syllabus Books
Text Book of Biochemistry 4th edition – U. Satyanarayana and Chakrapani.
Text Book of Medical Laboratory Technology 5th edition – Praful B. Godkar.
Text Book of Medical Laboratory Technology 5th edition – RamnikSood.
Text Book of Biochemistry for Medical Students 5th edition – DM Vasudevan.
Text Book of Medical Biochemistry 3rd edition. Dinesh Puri.
Reference Books & Readings
Victor W. Rodwell, David A. Bender. Harper’s Illustrated Biochemistry, 30th edition. A Lange Medical book (2015).
Richard Harvey, Denish Ferrier. Lippincott’s Illustrated Reviews Biochemistry 5th edition.
District Laboratory Practice in Tropical Countries 3rd edition (vol. 1) – Monika Cheesbrough.
District Laboratory Practice in Tropical Countries 3rd edition (vol. 2) – Monika Cheesbrough.
Manual of Laboratory & Diagnostic Tests – Mcgraw Hill.
Drew Provan, Andrew Krentz. Oxford Handbook of Clinical and Laboratory Investigation 2nd edition. Oxford University Press.