Syllabus | Cell biology | First Year | B.Sc Microbiology

Subject: Cell biology

S.no

Topic

Domain

Hours

1

An overview of cell and cell organelles:
Prokaryotic and eukaryotic cells, cell size and shape, molecules of cell, cell membranes and cell proteins., transport across nuclear envelope, The endoplasmic reticulum, golgi apparatus, lysosomes, mitochondria, chloroplast, peroxisomes.

Must know

14

Nuclear Envelope- structure of nuclear pore complex, nuclear lamina

Desirable to know

3

Chromatin: molecular organization, nucleolus and rRNA processing.

Nice to know

3

2

Cytoskeleton and cell movement:
Structure and organization of actin filaments; actin, myosin and cell movement; intermediate filaments; microtubules. Mechanism of vesicular transport. Endocytosis. Bacterial and Eukaryotic Cell Wall;

Must know

12

The plasma membrane structure; Transport of small molecules,

Desirable to know

4

the extracellular matrix and cell matrix interactions; cell-cell interactions.

Nice to know

4

3

Microscopic-Principles of Light microscopy; Phase contrast microscopy; Confocal microscopy; Electron microscopy (EM)- scanning EM and scanning transmission EM (STEM); Fluorescence microscopy.

Must know

10

Analytical- Flow cytometry- flurochromes, fluorescent probe and working principle;
Spectrophotometry; Mass spectrometry; X-ray diffraction analysis.

Desirable to know

5

Separation-Sub-cellular fractionation- differential and density gradient
centrifugation; Chromatography- paper, thin-layer, gel-filtration, ion-exchange,affinity and High-Performance Liquid Chromatography (HPLC)

Nice to know

5

4

Cell signaling, cell Cycle, cell death and renewal:
Signaling molecules and their receptor; functions of cell surface receptors;Cancer and mutation: Development and Causes of Cancer, Tumor Viruses, Oncogenes, Tumor Suppressor genes, Mutation, types of mutation.

Must know

10

Intracellular signal transduction pathway; signaling networks. Eukaryotic Cell Cycle, Regulation of Cell cycle progression, Events of Mitotic Phase, Meiosis and Fertilization. Programmed Cell Death, Stem Cells and Maintenance of adult tissues, Embryonic Stem Cells and Therapeutic cloning.

Desirable to know

8

Cancer Treatment- molecular approach.

Nice to know

2

Suggested readings:

  1. Karp, G. (2010). Cell and Molecular Biology: Concepts and Experiments. VI Edition. John Wiley & Sons. Inc.
  2. De Robertis, E.D.P. and De Robertis, E.M.F. (2006). Cell and Molecular Biology.
  3. VIII Edition. Lippincott Williams and Wilkins, Philadelphia.
  4. Cooper, G.M. and Hausman, R.E. (2009). The Cell: A Molecular Approach. V
  5. Edition. ASM Press & Sunderland, Washington, D.C.; Sinauer Associates, MA.
  6. Becker, W.M., Kleinsmith, L.J., Hardin. J. and Bertoni, G. P. (2009). The World of the Cell. VII Edition. Pearson Benjamin Cummings Publishing, San Francisco.
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