BIOCHEMISTRY 2nd YEAR CURRICULUM
| Sl. No | Contents of the Topics | Learning Objectives (At the end of the training the student must be able to) | Teaching Guidelines | Methodology | Time (Hours) |
| 1 | Structure of Cell | Understand the structure of cell | • Prokaryotic and Eukaryotic Cell
• Cell Membrane • Subcellular Organelles and their functions |
Lecture (using audio-visual aids) Demontration, Tutorial |
10 |
| 2 | Enzymology | Functioning and importance of enzymes
|
• Classification and mechanism of action of enzymes
• Factors affecting enzyme activity • Clinical importance of enzymes and iso-enzymes • Use of enzymes as reagents |
Lecture (using audio-visual aids) Demontration, Tutorial | 15 |
| 3 | Biochemistry of biomolecules | Basic Biochemistry of molecules | • Overview of biochemistry of – Carbohydrates, Lipids, Proteins, Nucleic Acids | Lecture (using audio-visual aids) Demontration, Tutorial |
15 |
| 4 | Organ function tests
|
Understand the normal functioning of vital organs of the body | • Kidney function tests
• Liver function tests • Thyroid function tests • Pancreatic function tests |
Lecture (using audio-visual aids) Demontration, Tutorial | 5 |
| 5 | Clinical Biochemistry
|
Explain the tools and techniques used in Clinical Biochemistry | • Specimen collection and processing
• Analytical variables • Glucose tolerance test • Electrophoresis • Chromatography • Biohazards in the laboratory • Porphyrias • Jaundice • Quality control • Preparing of reports |
Lecture (using audio-visual aids) Demontration, Tutorial |
20 |
MICROBIOLOGY 2nd YEAR CURRICULUM
| Sl. No | Contents of the Topics | Learning Objectives
(At the end of the training the student must be able to) |
Teaching Guidelines | Methodology | Time (Total-70) |
| 1 | Mycology
• Morphology • Classification of fungi • Cultivation of fungi • Stains used in mycology |
Reproduce general morphological characteristics of fungi, Classification of fungi and its cultivation method.
Students should be able to demonstrate staining methods used in mycology |
The topic should cover general morphological characteristics of fungi, Classification of fungi and its cultivation method.
Students should be able to demonstrate staining methods used in mycology
|
Didactics, video and practical demonstration | 4 |
| 2 | Lab diagnosis of fungal Infections
Superficial Mycoses: Dermatophytes, Candidiasis. |
Perform Lab diagnosis of fungal Infections which includes
Superficial Mycoses:-Dermatophytes and Candidiasis. |
Topic should cover Lab diagnosis of fungal Infections which includes
Superficial Mycoses:-Dermatophytes and Candidiasis. |
Didactics, video and presentation | 3 |
| 3 | Subcutaneous Mycoses:
Mycetoma, Rhinosporidium, and Sporotrichosis |
Subcutaneous Mycoses:
Mycetoma, Rhinosporidium, and sporotrichosis |
Subcutaneous Mycoses:
Mycetoma, Rhinosporidium, and sporotrichosis |
Didactics, video and presentation | 3 |
| 4 | Systemic Mycoses:
Histoplasmosis, Blastomycosis Cryptococcosis |
Systemic Mycoses:
Histoplasmosis, Blastomycosis Cryptococcosis
|
Systemic Mycoses:
Histoplasmosis, Blastomycosis Cryptococcosis
|
Diadetics, video and presentation | 3 |
| 5 | Opportunistic Fungi:
Aspergillosis, Pencillosis, Zygomycosis, Pneumocystis |
Opportunistic Fungi:
Aspergillosis, Pencillosis, Zygomycosis, Pneumocystis |
Opportunistic Fungi:
Aspergillosis, Pencillosis, Zygomycosis, Pneumocystis |
Diadetics, video and presentation | 3 |
| 6 | Parasitology
Classification and morphology of Protozoa Classification and morphology and Helminthes |
Reproduce classification of protozoa, heminthes. The students should learn general morphological characteristic of protozoa and Helminthes.
|
Topic should cover classification of protozoa, heminthes. The students should learn general morphological characteristic of protozoa and Helminthes.
|
Didactics, video and presentation | 4 |
| 7 | Structure, life cycle pathogenesis & laboratory diagnosis of following parasites
Protozoa : Entamoeba, Trichomonas and Giardia, Plasmodium, Lieshmania Toxoplasma, Cryptosporidium & other coccidian parasites. |
Explain Structure, life cycle pathogenesis & laboratory diagnosis of following parasites
Protozoa : Entamoeba, Trichomonas and Giardia, Plasmodium, Lieshmania Toxoplasma, Cryptosporidium & other coccidian parasites. |
Topic should cover Structure, life cycle pathogenesis & laboratory diagnosis of following parasites
Protozoa : Entamoeba, Trichomonas and Giardia, Plasmodium, Lieshmania Toxoplasma, Cryptosporidium & other coccidian parasites. |
Didactics, students seminar and practical demonstration | 8 |
| 8 | Helminthes :
Taenia, Echinococcus. Ascaris, Ancylostoma, Strongyloides, Trichuris, &Enterobius, Filaria |
Helminthes :
Taenia, Echinococcus. Ascaris, Ancylostoma, Strongyloides, Trichuris, &Enterobius, Filaria |
Helminthes :
Taenia, Echinococcus. Ascaris, Ancylostoma, Strongyloides, Trichuris, &Enterobius, Filaria |
Didactics, video and presentation | 8
|
| 9 | Virology
General properties of Viruses Collection, transportation and storage of samples for viral diagnosis. Cultivation of viruses. Bacteriophages |
Demonstrate
General properties of Viruses method of Collection, transportation and storage of samples for viral diagnosis. Cultivation of viruses. Explain life cycle of Bacteriophages (lysogenic and lytic) |
Topic covers
General properties of Viruses method of Collection, transportation and storage of samples for viral diagnosis. Cultivation of viruses. Bacteriophages (lysogenic and lytic) |
Seminar and group discussion | 5 |
| 10 | Disease caused (pathogenesis), lab diagnosis, and prevention of:
Herpes viruses ,
Viral Hepatitis, uman Immunodeficiency Viruses , Rabies, Poliomyelitis, Influenza Viruses Rubella, Mumps Measles Rota virus. Japanese encephalitis & Dengue, Chikungunya, Kyasanur Forest disease Human Onocogenic Viruses.
|
Reproduce the Disease caused (pathogenesis), lab diagnosis, and prevention of given viruses
Herpes viruses (type 1 and 2) Viral Hepatitis (A and B) Human Immunodeficiency Viruses Rabies, Poliomyelitis, Influenza Viruses Rubella, Mumps , Measles Rota virus. Japanese encephalitis &
Dengue, Chikungunya, Kyasanur Forest disease Human Oncogenic Viruses. (Definition, infectious causes of cancer; pathogenesis, lab diagnosis and prevention of retrovirus, papovaviridae) |
Topic should cover Disease caused (pathogenesis), lab diagnosis, and prevention of given viruses
Herpes viruses (type 1 and 2) Viral Hepatitis (A and B) Human Immunodeficiency Viruses Rabies, Poliomyelitis, Influenza Viruses Rubella, Mumps , Measles Rota virus. Japanese encephalitis &Dengue, Chikungunya, Kyasanur Forest disease
Human Oncogenic Viruses. (Definition, infectious causes of cancer; pathogenesis, lab diagnosis and prevention of retrovirus, papovaviridae) |
Seminar, presentation, video, practical demonstration for lab diagnosis wherever applicable | 12 |
| 11 | Specimen processing
|
Perform microbiological processing of the given clinical samples
CSF and other body fluids, Stool and rectal swabs |
Topic must cover microbiological processing of the given clinical samples
CSF and other body fluids, Stool and rectal swabs |
Student interaction, video, practical demonstration | 5 |
| 12 | Applied Microbiology
Hospital infection control Health care associated infection Emerging infectious diseases |
Implement Hospital infection control methods. Control methods in terms of lineal sample handling and general safety measures in hospitals and labs. They should also have understanding of quality control and quality assurance audits.
Students should be able to understand Health care associated infection like blood borne infections (HIV, Hepatitis); respiratory infections like tuberculosis and due to pseudomonas.
Students should also know the pathogenesis, lab diagnosis and prevention of Emerging infectious diseases like H1N1 and SARS.
|
The topic should over Hospital infection control methods. Control methods in terms of lineal sample handling and general safety measures in hospitals and labs. They should also have understanding of quality control and quality assurance audits.
Students should be able to understand Health care associated infection like blood borne infections (HIV, Hepatitis); respiratory infections like tuberculosis and due to pseudomonas.
Students should also know the pathogenesis, lab diagnosis and prevention of Emerging infectious diseases like H1N1 and SARS. |
Student interaction, video, practical demonstration | 8 |
PATHOLOGY 2nd YEAR CURRICULUM
| Sl. No | Contents of the Topics | Learning Objectives
(At the end of the training the student must be able to) |
Teaching Guidelines | Methodology | Time |
| Fundamentals of applied histology
Microscopy – working principle, maintenance and applications, & various types of microscope.
Dark ground microscope, Polarizing microscope, Phase contrast microscope, interference microscope, U.V light microscope. H&E Stain & its importance. Connective tissue stain, trichrome staining and other special stains. Principle of metal impregnation techniques. Principles of immunohistochemistry and its techniques. |
Demonstrate the Structure and principle of :Light microscope, Dark field, Phase contrast microscopy, Fluorescent, Polarizing microscope, & Electron microscopy
They able to demonstrate methods & principle of H&E staining, trichrome staining, PAS, metal impregnation techniques and Immunohistochemistry
|
The topic should coverStructure and principle of :Light microscope, Dark field, Phase contrast microscopy, Polarizing microscope, Fluorescent & Electron microscopy
It also cover procedure of staining H&E, PAS, MT, Reticuline, Immunohistochemistry
|
Didactic
Student interactive session
Seminar
|
12hr |
|
| Cytology
Stains cytological preparation with special emphasis on MGG, Papanicoclaos’s Stains. Special stains like PAS, Mucicarmine, Alcian blue. Cytological screening and quality control in cytology laboratory. |
Demonstrate method & principles of MGG, Papanicoclaos’s and H&E, PAS, Mucicarmine, Aician blue
They able to perform cytological screening programs and quality control in cytology laboratory |
The topic should cover method of stain preparation and method of staining MGG, Papanicoclaos’s and H&E, PAS, Mucicarmine, Aician blue
It also cover cervical screening method & quality control in cytology laboratory |
Didactic
Student interactive session
|
08hr |
|
| Haematology
Haematopoiesis& stem cell.
Anaemia’s:- Types, classification, definition & microcytic hypochromic & macrocytic anemia
Bone marrow aspiration composition and function Staining of bone marrow smears and preparation of histological section
Haemoglobin:- Its synthesis, functions and degradation
Haemoglobin pigments and their measurement Abnormal haemoglobins and their means of identification & estimation LE Cell phenomenon, and various methods of its demonstration.
Coagulation factors.
Haemostatic mechanism and theories of blood coagulation &Haemophilia
Preparation of packed cells and various fraction of blood for transfusion purposes |
Demonstratehaematopoiesis& stem cell , types of anaemia, their causes, clinical features & their pathological findings
They should be able to demonstrate bone marrow staining & preparation of histological section
They also able to demonstrate structure & function of normal &abnormal Haemoglobin, their degradation & measurement
At the end of this teaching the students should be able to demonstrate LE cell & method of its demonstration
Elaboratevarious coagulative factors, their haemostatic mechanism and coagulation disorders
Perform Preparation of packed cells and various fraction of blood for transfusion purposes |
The topic should cover iron deficiency anaemia, megaloblastic anaemia, haemolyticanaemia and their pathogenesis.
It also covers bone marrow aspiration smear prepararation and their staining methods. It also included abnormal haemoglobin like alpha, beta, delta-beta thalassemia, HB C, D and sickle Hb.
It also covers causes of LE cells and its principle
It should covervarious coagulative factors (II, VII, VIII, V, X, XI, XII), Coagulation pathway- Intrinsic, Extrinsic & common pathway, Coagulation disorders- Haemophilia A&B, VWB disease It should cover preparation of PRC,FFP, PRP, SDP and storage temperature |
Didactic
Seminar
Group discussion
|
25hr |
|
|
General Pathology
Inflammation:- Definition, causes, types & various cells of inflammation.
Immunity:- Definition, types of antigens & various types of antibodies. Hypersensitivity:- Definition with types & examples.
Neoplasia:- Definition, classification, difference between benign & malignant tumors in brief, various modes of invasion and diagnosis in brief.
Infections:- Malaria, tuberculosis, dengue & AIDS in brief.
Nutritional diseases:- Fat & water soluble vitamins, Rickets, Scurvy |
Demonstrate & know about mechanism of inflammation, type of inflammation, causes of inflammation, various cells of inflammation
At the end of this teaching the students should be able to demonstrate – Mechanism of immunity types of antigens & various types of antibodies, types of hypersensitivity reactions & their manifestations
Demonstrate & know the classification, difference between benign & malignant tumors, various modes of metastasis & diagnostic features of tumors
Demonstrate causative agents of malaria, tuberculosis, dengue & AIDS, their pathogenesis and their clinical manifestations.
Demonstrate the use & significance of fat & water soluble vitamins, pathogenesis & clinical manifestations of Rickets & Scurvy |
The topic should cover Definition, Causes (Infection, trauma, Tissue necrosis ,Foreign bodies,Immune reactions),
Types (Acute & Chronic) & Various cells of inflammation (neutrophils, Mast cells, basophils, platelets,Macrophages, endothelial cells). The topic should cover Definition of immunity, Antigens ( self& non-self ), Antibodies (IgG, IgA, IgM, IgE, IgD). Type of hypersensitivity reactions & their examples -immediate hypersensitivity (type I),antibody-mediated disorders (type II),immune complex–mediated disorders (type III),cell-mediated immune disorders (type IV).
The topic should cover- Definition of neoplasia, classification neoplasia, difference between benign( lipoma, fibroadenoma) & malignant tumors( carcinoma, adenocarcinoma, sarcoma), modes of metastasis( direct invasion,hematogenous spread & lymphatic spreads). It should cover pathological findings(gross& microscopic) of Malaria, Tuberculosis, Dengue & AIDS It should covers- fat ( A, D, E, & K) and water(C, B complex) soluble vitamins, pathological findings(gross& microscopic) of Rickets & Scurvy |
Didactic
Seminar
Group discussion
|
15hr |
|
|
Fundamentals of transfusion Medicine: Compatibility of tests in blood transfusion.
Complications and hazard of blood transfusion Laboratory investigation of transfusion reactions and mismatched transfusion. Various component of blood:- Separation & its uses. |
Demonstrate & know Compatibility& incompatibility of tests in blood transfusion, complications of blood transfusion and laboratory investigation of transfusion reactions, & mismatched transfusion. They should be able to demonstrate preparations of various blood components and their uses. |
The topic should cover- transfusion reactions (immune mediated & nonimmune type reactions) and immediate type & delayed type reactions, other complications –iron overload, hyper & hypo potassium, fluid overload.
Laboratory investigations included ICT, DCT, microbiological, PBF for hemolysis, LDH, bilirubin, urine blood testing. Procedure of PRC, FFP, PRP, SDP preparations. |
Seminar Didactic
Student interactive session |
10hr |