CURRICULUM | DIPLOMA MEDICAL LABORATORY TECHNOLOGY | SECOND YEAR

BIOCHEMISTRY  2nd  YEAR CURRICULUM

 

Sl. No Contents of the Topics Learning Objectives (At the end of the training the student must be able to) Teaching Guidelines Methodology Time (Hours)
1 Structure of Cell Understand the structure of cell •         Prokaryotic and Eukaryotic Cell

•         Cell Membrane

•         Subcellular Organelles and their functions

Lecture (using audio-visual aids) Demontration, Tutorial  

10

2 Enzymology Functioning and importance of enzymes

 

•         Classification and mechanism of action of enzymes

•         Factors affecting enzyme activity

•         Clinical importance of enzymes and iso-enzymes

•         Use of enzymes as reagents

Lecture (using audio-visual aids) Demontration, Tutorial 15
3 Biochemistry of biomolecules Basic Biochemistry of molecules •         Overview of biochemistry of – Carbohydrates, Lipids, Proteins, Nucleic Acids Lecture (using audio-visual aids) Demontration, Tutorial  

 

 

15

4 Organ function tests

 

Understand the normal functioning of vital organs of the body •         Kidney function tests

•         Liver function tests

•         Thyroid function tests

•         Pancreatic function tests

Lecture (using audio-visual aids) Demontration, Tutorial 5
5 Clinical Biochemistry

 

Explain the tools and techniques used in Clinical Biochemistry •         Specimen collection and processing

•         Analytical variables

•         Glucose tolerance test

•         Electrophoresis

•         Chromatography

•         Biohazards in the laboratory

•         Porphyrias

•         Jaundice

•         Quality control

•         Preparing of reports

Lecture (using audio-visual aids) Demontration, Tutorial  

 

 

20

 

MICROBIOLOGY 2nd YEAR CURRICULUM

 

Sl. No Contents of the Topics Learning Objectives

(At the end of the training the student must be able to)

Teaching Guidelines Methodology Time (Total-70)
1 Mycology

•         Morphology

•         Classification of fungi

•         Cultivation of fungi

•            Stains used in mycology

Reproduce general morphological characteristics of fungi,                         Classification of fungi                                                                                    and its cultivation method.

 

Students should be able to demonstrate staining methods used in mycology

The topic should cover general morphological characteristics of fungi,                         Classification of fungi                                                                                    and its cultivation method.

Students should be able to demonstrate staining methods used in mycology

 

Didactics, video and practical demonstration 4
2 Lab diagnosis of fungal Infections

Superficial Mycoses:

Dermatophytes,

Candidiasis.

Perform Lab diagnosis of fungal Infections which includes

Superficial Mycoses:-Dermatophytes and                                                                                       Candidiasis.

Topic should cover Lab diagnosis of fungal Infections which includes

Superficial Mycoses:-Dermatophytes and                                                                                       Candidiasis.

Didactics, video and presentation 3
3 Subcutaneous Mycoses:

Mycetoma,

Rhinosporidium, and  Sporotrichosis

Subcutaneous Mycoses:

 

Mycetoma,                                                                                                Rhinosporidium, and  sporotrichosis

Subcutaneous Mycoses:

Mycetoma,                                                                                                Rhinosporidium, and  sporotrichosis

Didactics, video and presentation 3
4 Systemic Mycoses:

Histoplasmosis, Blastomycosis

Cryptococcosis 

Systemic Mycoses:

Histoplasmosis, Blastomycosis

Cryptococcosis

 

Systemic Mycoses:

 

Histoplasmosis, Blastomycosis

Cryptococcosis

 

Diadetics, video and presentation 3
5 Opportunistic Fungi:

Aspergillosis, Pencillosis,

Zygomycosis, Pneumocystis

Opportunistic Fungi:

Aspergillosis, Pencillosis,

Zygomycosis, Pneumocystis

Opportunistic Fungi:

Aspergillosis, Pencillosis,

Zygomycosis, Pneumocystis

Diadetics, video and presentation 3
6 Parasitology

Classification and morphology of Protozoa

Classification and morphology and Helminthes

Reproduce classification of protozoa, heminthes. The students should learn general morphological characteristic of protozoa and Helminthes.

 

Topic should cover classification of protozoa, heminthes. The students should learn general morphological characteristic of protozoa and Helminthes.

 

Didactics, video and presentation 4
7 Structure, life cycle pathogenesis &  laboratory diagnosis of following parasites

 

Protozoa :

Entamoeba,

Trichomonas and Giardia,

Plasmodium,

Lieshmania

Toxoplasma,

Cryptosporidium & other coccidian parasites.

Explain Structure, life cycle pathogenesis &  laboratory diagnosis of following parasites

Protozoa :

Entamoeba,

Trichomonas and Giardia,

Plasmodium,

Lieshmania

Toxoplasma,

Cryptosporidium & other coccidian parasites.

Topic should cover Structure, life cycle pathogenesis &  laboratory diagnosis of following parasites

Protozoa :

Entamoeba,

Trichomonas and Giardia,

Plasmodium,

Lieshmania

Toxoplasma,

Cryptosporidium & other coccidian parasites.

Didactics, students seminar and practical demonstration 8
8 Helminthes :

Taenia,

Echinococcus.

Ascaris,

Ancylostoma, Strongyloides,

Trichuris, &Enterobius,

Filaria

Helminthes :

Taenia,

Echinococcus.

Ascaris,

Ancylostoma, Strongyloides,

Trichuris, &Enterobius,

Filaria

Helminthes :

Taenia,

Echinococcus.

Ascaris,

Ancylostoma, Strongyloides,

Trichuris, &Enterobius,

Filaria

Didactics, video and presentation 8

 

 

 

 

9 Virology

General properties of Viruses

Collection, transportation and storage of samples for viral diagnosis.

Cultivation of viruses.  Bacteriophages

Demonstrate

General properties of Viruses                                                                                     method of Collection, transportation and storage of samples for viral diagnosis. Cultivation of viruses.                                                                                                   Explain life cycle of Bacteriophages (lysogenic and lytic)

Topic covers

General properties of Viruses                                                                                     method of Collection, transportation and storage of samples for viral diagnosis. Cultivation of viruses.

Bacteriophages (lysogenic and lytic)

Seminar and group discussion 5
10 Disease caused (pathogenesis), lab diagnosis, and prevention of:

 

Herpes viruses ,

 

Viral Hepatitis,

uman Immunodeficiency Viruses ,

Rabies,

Poliomyelitis,

Influenza Viruses

Rubella, Mumps Measles

Rota virus.

Japanese encephalitis & Dengue,

Chikungunya, Kyasanur Forest disease

Human Onocogenic Viruses.

 

 

Reproduce the Disease caused (pathogenesis), lab diagnosis, and prevention of given viruses

Herpes viruses (type 1 and 2)

Viral Hepatitis (A and B)

Human Immunodeficiency Viruses

Rabies,

Poliomyelitis,

Influenza Viruses

Rubella, Mumps , Measles

Rota virus.

Japanese encephalitis &

 

Dengue,

Chikungunya, Kyasanur Forest disease

Human Oncogenic Viruses. (Definition, infectious causes of cancer; pathogenesis, lab diagnosis and prevention of retrovirus, papovaviridae)

Topic should cover Disease caused (pathogenesis), lab diagnosis, and prevention of given viruses

Herpes viruses (type 1 and 2)

Viral Hepatitis (A and B)

Human Immunodeficiency Viruses

Rabies,

Poliomyelitis,

Influenza Viruses

Rubella, Mumps , Measles

Rota virus.

Japanese encephalitis &Dengue,

Chikungunya, Kyasanur Forest disease

 

Human Oncogenic Viruses. (Definition, infectious causes of cancer; pathogenesis, lab diagnosis and prevention of retrovirus, papovaviridae)

Seminar, presentation, video, practical demonstration for lab diagnosis wherever applicable 12
11 Specimen processing

  • Blood
  • Sputum, throat swab, nasopharyngeal swab, Swabs (pus, wound )
  • CSF and other body fluids, Stool and rectal swabs.

 

 

Perform microbiological processing of the given clinical samples

  • Blood
  • Sputum, throat swab, nasopharyngeal swab, Swabs (pus, wound )

CSF and other body fluids, Stool and rectal swabs

Topic must cover microbiological processing of the given clinical samples

  • Blood
  • Sputum, throat swab, nasopharyngeal swab, Swabs (pus, wound )

CSF and other body fluids, Stool and rectal swabs

Student interaction, video, practical demonstration 5
12 Applied Microbiology

Hospital infection control

Health care associated infection

Emerging infectious diseases

Implement Hospital infection control   methods. Control methods in terms of lineal sample handling and general safety measures in hospitals and labs. They should also have understanding of quality control and quality assurance audits.

 

Students should be able to understand

Health care associated infection like blood borne infections (HIV, Hepatitis); respiratory infections like tuberculosis and due to pseudomonas.

 

Students should also know the pathogenesis, lab diagnosis and prevention of

Emerging infectious diseases like H1N1 and SARS.

 

The topic should over Hospital infection control   methods. Control methods in terms of lineal sample handling and general safety measures in hospitals and labs. They should also have understanding of quality control and quality assurance audits.

Students should be able to understand

Health care associated infection like blood borne infections (HIV, Hepatitis); respiratory infections like tuberculosis and due to pseudomonas.

 

Students should also know the pathogenesis, lab diagnosis and prevention of

Emerging infectious diseases like H1N1 and SARS.

Student interaction, video, practical demonstration 8

 

 

PATHOLOGY 2nd YEAR CURRICULUM

 

Sl. No Contents of the Topics Learning Objectives

(At the end of the training the student must be able to)

Teaching Guidelines Methodology Time
Fundamentals of applied histology

 

Microscopy – working principle, maintenance and applications, & various types of microscope.

 

Dark ground microscope, Polarizing microscope, Phase contrast microscope, interference microscope, U.V light microscope.

H&E Stain & its importance.

Connective tissue stain, trichrome staining and other special stains.

Principle of metal impregnation techniques.

Principles of immunohistochemistry and its techniques.

 

Demonstrate the Structure and principle of :Light microscope, Dark field, Phase contrast microscopy, Fluorescent, Polarizing microscope,  & Electron microscopy

 

 

 

 

 

They able to demonstrate methods & principle of H&E staining, trichrome staining, PAS, metal impregnation techniques and Immunohistochemistry

 

 

The topic should coverStructure and principle of :Light microscope, Dark field, Phase contrast microscopy, Polarizing microscope, Fluorescent & Electron microscopy

 

 

 

 

 

It also cover procedure of staining H&E, PAS, MT, Reticuline, Immunohistochemistry

 

 

 

 

 

 

 

Didactic

 

 

 

Student interactive session

 

 

 

Seminar

 

 

 

 

 

 

 

 

12hr

Cytology

Stains cytological preparation with special emphasis on MGG, Papanicoclaos’s Stains.

Special stains like PAS, Mucicarmine, Alcian blue.

Cytological screening and quality control in cytology laboratory.

 

Demonstrate method & principles of  MGG, Papanicoclaos’s and H&E, PAS, Mucicarmine, Aician blue

 

 

 

They able to perform cytological screening programs and quality control in cytology laboratory

The topic should cover method of stain preparation and method of staining MGG, Papanicoclaos’s and H&E, PAS, Mucicarmine, Aician blue

 

 

 

It also cover cervical screening method & quality control in cytology laboratory

 

 

 

 

 

 

Didactic

 

Student interactive session

 

 

 

 

 

 

 

08hr

Haematology

 Haematopoiesis& stem cell.

 

Anaemia’s:- Types, classification, definition & microcytic hypochromic & macrocytic anemia

 

Bone marrow aspiration composition and function

Staining of bone marrow smears and preparation of histological section

 

Haemoglobin:- Its synthesis, functions and degradation

 

Haemoglobin pigments and their measurement

Abnormal haemoglobins and their means of identification & estimation

LE Cell phenomenon, and various methods of its demonstration.

 

Coagulation factors.

 

Haemostatic mechanism and theories of blood coagulation &Haemophilia

 

Preparation of packed cells and various fraction of blood for transfusion purposes

 

 

Demonstratehaematopoiesis& stem cell , types of anaemia, their causes, clinical features & their pathological findings

 

 

They should be able to demonstrate  bone marrow staining & preparation of histological section

 

They also able to demonstrate structure & function of normal &abnormal Haemoglobin, their degradation & measurement

 

 

 

At the end of this teaching the students should be able to demonstrate LE cell & method of its demonstration

 

Elaboratevarious coagulative factors, their haemostatic mechanism and coagulation disorders

 

 

 

 

Perform Preparation of packed cells and various fraction of blood for transfusion purposes

 

 

The topic should cover iron deficiency anaemia, megaloblastic anaemia, haemolyticanaemia and their pathogenesis.

 

 

It also covers bone marrow aspiration smear prepararation and their staining methods.

It also included abnormal haemoglobin like alpha, beta, delta-beta thalassemia, HB C, D and sickle Hb.

 

 

 

 

 

 

 

It also covers causes of LE cells and its principle

 

 

It should covervarious coagulative factors (II, VII, VIII, V, X, XI, XII),

Coagulation pathway- Intrinsic, Extrinsic & common pathway,

Coagulation disorders- Haemophilia A&B, VWB disease

It should cover preparation of PRC,FFP, PRP, SDP and storage temperature

 

 

 

 

 

 

 

 

 

 

 

Didactic

 

 

 

 

 

Seminar

 

 

 

 

 

 

 

 

 

Group discussion

 

 

 

 

25hr

 

General Pathology

 

Inflammation:- Definition, causes, types & various cells of inflammation.

 

 

Immunity:-  Definition, types of antigens & various types of antibodies.

Hypersensitivity:- Definition with types & examples.

 

 

 

Neoplasia:- Definition, classification, difference between benign & malignant tumors in brief, various modes of invasion and diagnosis in brief.

 

Infections:- Malaria, tuberculosis, dengue & AIDS in brief.

 

Nutritional diseases:-  Fat & water soluble vitamins, Rickets, Scurvy

 

Demonstrate & know about mechanism of inflammation,  type of inflammation, causes of inflammation, various cells of inflammation

 

 

At the end of this teaching the students should be able to demonstrate – Mechanism of immunity  types of antigens & various types of antibodies, types of hypersensitivity reactions & their manifestations

 

 

 

 

 

Demonstrate & know the classification, difference between benign & malignant tumors, various modes of metastasis & diagnostic features of tumors

 

Demonstrate causative agents of malaria, tuberculosis, dengue & AIDS, their pathogenesis and their clinical manifestations.

 

Demonstrate the use & significance of fat & water soluble vitamins, pathogenesis & clinical manifestations of Rickets & Scurvy

 The topic should cover Definition, Causes (Infection, trauma, Tissue necrosis ,Foreign bodies,Immune reactions),

Types (Acute & Chronic) & Various cells of inflammation (neutrophils, Mast cells, basophils, platelets,Macrophages, endothelial cells).

The topic should cover Definition of immunity, Antigens ( self& non-self ), Antibodies (IgG, IgA, IgM, IgE, IgD).

Type of hypersensitivity reactions & their examples -immediate hypersensitivity (type I),antibody-mediated disorders (type II),immune complex–mediated disorders (type III),cell-mediated immune disorders (type IV).

 

The topic should cover- Definition of neoplasia, classification neoplasia, difference between benign( lipoma, fibroadenoma) & malignant tumors( carcinoma, adenocarcinoma, sarcoma), modes of metastasis( direct invasion,hematogenous spread & lymphatic spreads).

It should cover pathological findings(gross& microscopic) of Malaria, Tuberculosis, Dengue & AIDS

It should covers- fat ( A, D, E, & K) and water(C, B complex) soluble vitamins, pathological findings(gross& microscopic) of Rickets & Scurvy

 

 

 

Didactic

 

 

 

 

 

 

 

 

 

Seminar

 

 

 

 

 

Group discussion

 

 

 

 

 

 

 

 

 

 

15hr

 

Fundamentals of transfusion Medicine:

Compatibility of tests in blood transfusion.

 

Complications and hazard of blood transfusion

Laboratory investigation of transfusion reactions and mismatched transfusion.

Various component of blood:- Separation & its uses.

 

 

  

 

Demonstrate & know Compatibility&  incompatibility of tests in blood transfusion, complications of blood transfusion and laboratory investigation of transfusion reactions, & mismatched transfusion.

They should be able to demonstrate preparations of various blood components and their uses.

The topic should cover- transfusion reactions (immune mediated & nonimmune type reactions) and immediate type & delayed type reactions, other complications –iron overload, hyper & hypo potassium, fluid overload.

Laboratory investigations included ICT, DCT, microbiological, PBF for hemolysis, LDH, bilirubin, urine blood testing.

Procedure of PRC, FFP, PRP, SDP preparations.

 

 

 

 

 

 

Seminar

Didactic

 

 

 

Student interactive session

 

 

 

 

 

10hr

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