BMLT | MICROBIOLOGY | Third Year

(Total-70)

Microbial pathogenicity

Immunity:

Innate immunity

Acquired immunity(adaptive immunity) Active and passive immunity

4

Organs of immune system

4

Macrophages and dendritic cells

Humoral immunity

i)             Cell-mediated immunity

Antigens

Types of antibodies  and classes of immunoglobulins

Precipitation, Agglutination

Complement Fixation Test, Neutralization

ELISA,

Immunofluorescence ,Radioimmunoassay,

Opsonisation

Must know

Nice to know

Must know

2

1

1

Classification, Morphology, Genotypic &Phenotypic characteristics, Pathogenesis Disease caused, Lab Diagnosis &Prophylaxis of Rare pathogens

Mycoplasma, Chlamydia,

Legionella,

Actinomycosis&Nocordia

Rickettsia

Must know

Nice to know

Must know

4

2

1

Bacteriology of Water, Milk,  and Air Laboratory Diagnosis of

                      –  Urinary Tract Infections

–  Diarrhea & Dysentery

–  Meningitis

–  Blood stream infection

–  Respiratory infection

–  Sexually Transmitted Diseases

–  Viral hepatitis

–  HIV

–  Skin, soft tissue & wound infection

Desirable to know

Must know

Nice to know

Must know

Must know

Must know

Desirable to know

Must know

Desirable to know

1

1

1

1

2

1

1

1

1

DNA hybridization etc

Desirable to know

3

i)       Grams staining

ii)     ZN staining

iii)   Albert’s staining

Hanging drop preparation

Culture methods

I.            Culture media

II.            Culture techniques

Introduction to biochemical reactions

Identification of bacterial culture

i)       Colony characteristics

ii)     Morphological characteristics

iii)   Motility study

Interpretation of biochemical reactions

Antibiotic sensitivity testing-Kirby Bauer method

ImmunologySerologicaltests

i)             Specimen collection, Principle, Methods, Procedure

ii)           Demonstration of HIV, HCV, HBC, Dengue,

iii)         Rapid test for Malaria,

iv)         Demonstration of ASO, CRP, RA, Widal, VDRL, Typhidot

Biomedical Waste management