BMLT | MICROBIOLOGY | Third Year

Syllabus (MICROBIOLOGY paper III)

S. No Topic Domain Hours

(Total-70)

1 Immunology

Microbial pathogenicity

Immunity:

Innate immunity

Acquired immunity(adaptive immunity) Active and passive immunity

Must know  

 

4

2

 

Components of immune system :

Organs of immune system

Must know  

 

4

B Lymphocytes and plasma cells Must know
T lymphocytes and their subsets and Natural killer cells

Macrophages and dendritic cells

Must know
3 Immune response

Humoral immunity

i)             Cell-mediated immunity

Must know 2
Must know 2
4 Types of hypersensitivity reactions Desirable to know 2
5 Autoimmunity Desirable to know 2
6 Cytokines Nice to know 1
7 Antigen &Antibody

Antigens

Types of antibodies  and classes of immunoglobulins

Must know 4
8 Complement Desirable to know 2
9 Types of antigen-antibody reactions

Precipitation, Agglutination

Complement Fixation Test, Neutralization

ELISA,

Immunofluorescence ,Radioimmunoassay,

Opsonisation

 

 

Must know

Nice to know

Must know

 

 

2

1

1

10 Monoclonal Antibodies Must know 2
11 Transplantation immunology and HLA typing Nice to know 3
12 Systematic Bacteriology

Classification, Morphology, Genotypic &Phenotypic characteristics, Pathogenesis Disease caused, Lab Diagnosis &Prophylaxis of Rare pathogens

Mycoplasma, Chlamydia,

Legionella,

Actinomycosis&Nocordia

Rickettsia

 

Must know

 

Nice to know

 

Must know

 

4

 

2

 

1

13 Applied Microbiology

Bacteriology of Water, Milk,  and Air Laboratory Diagnosis of

                      –  Urinary Tract Infections

–  Diarrhea & Dysentery

–  Meningitis

–  Blood stream infection

–  Respiratory infection

–  Sexually Transmitted Diseases

–  Viral hepatitis

–  HIV

–  Skin, soft tissue & wound infection

 

Desirable to know

Must know

Nice to know

Must know

Must know

Must know

Desirable to know

Must know

Desirable to know

 

1

1

1

1

2

1

1

1

1

14 Molecular techniques in diagnostic microbiology-PCR,

DNA hybridization etc

Must know

Desirable to know

2

3

15 Biomedical waste management Desirable to know 2
16 Biosafety levels and biosafety cabinets Must know 2
17 Health care associated infections Must know 4
18 Universal Precautions Nice to know 1
17 Occupationally acquired infections in health care settings Must know 4
18 Vaccination for health care personnel Must know 2
19 Maintenance of laboratory records, Audit Nice to know 1

 

Syllabus (Microbiology practical paper III)

S. No Topic Domain Hours
1. Staining

i)       Grams staining

ii)     ZN staining

iii)   Albert’s staining

Hanging drop preparation

Culture methods

I.            Culture media

II.            Culture techniques

Introduction to biochemical reactions

Identification of bacterial culture

i)       Colony characteristics

ii)     Morphological characteristics

iii)   Motility study

Interpretation of biochemical reactions

Antibiotic sensitivity testing-Kirby Bauer method

ImmunologySerologicaltests

i)             Specimen collection, Principle, Methods, Procedure

ii)           Demonstration of HIV, HCV, HBC, Dengue,

iii)         Rapid test for Malaria,

iv)         Demonstration of ASO, CRP, RA, Widal, VDRL, Typhidot

Must know 20
2. Applied

Biomedical Waste management

Desirable to know 5
3. Maintenance of equipment Nice to know 5
SGTUNIVERSITY
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