BMLT | MICROBIOLOGY | Second Year

Morphology

Classification of fungi

Cultivation of fungi

stains used in mycology

Desirable to know

Nice to know

Must know

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Superficial Mycoses:

1. Dermatophytes,
2. Candidiasis.

1. Mycetoma,
2. Rhinosporidium, and Sporotrichosis

1. Histoplasmosis, Blastomycosis
2. Cryptococcosis

• Aspergillosis, Pencillosis, 
• Zygomycosis,
• Pneumocystis

Nice to know

Must know

1.      Classification and morphology of Protozoa Classification and morphology and Helminthes

Must know 

Nice to know

i.            Protozoa :

a)      Entamoeba,

b)      Trichomonas and Giardia,

c)      Plasmodium,

d)      Lieshmania

e)      Toxoplasma,

f)       Cryptosporidium & other coccidian parasites.

Must know

Nice to know

 Must know

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a)         Taenia,

b)         Echinococcus.

c)         Ascaris,

d)         Ancylostoma, Strongyloides,

e)         Trichuris, &Enterobius, Filaria

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1. General properties of Viruses
2. Collection, transportation and storage of samples for viral diagnosis.
3. Cultivation of viruses.
4. Bacteriophages

Must know

Desirable to know

Nice to know

1. Herpes viruses ,
2. Viral Hepatitis,
3. Human Immunodeficiency Viruses ,
4. Rabies.
5. Poliomyelitis,
6. Influenza Viruses
7. Rubella, Mumps , Measles
8. Rota virus.
9. Japanese encephalitis & Dengue,
10. Chikungunya
11. Kyasanur Forest disease
12. Human Onocogenic Viruses.

Must know

Nice to know

Desirable to know

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• Blood
• Sputum, throat swab, nasopharyngeal swab, Swabs (pus, wound )
• CSF and other body fluids,
• Stool and rectal swabs.

Must know

Desirable to know

Must know

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Hospital infection control

Health care associated infection

Emerging infectious diseases

Must know

Desirable to know

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(Total-30)

i)       Demonstration of fungi using KOH , Lactophenol cotton blue and India ink

ii)     Colony characteristics and Microscopic examination and identification tests for :

iii)   Candida and  Cryptococcus,

iv)   Dermatophytes

v)      Aspergillussp

Parasitology

I.            Stool examination: Saline mount, Iodinemount

II.            Stool concentration techniques

III.            Preparation of thick and thin smears

Spottests/ELISA: HBV, HCV,HIV, Dengue X2

vii) Slide culture technique

I.            Preparation and staining technique of Leishman’s stain and Giemsa stain

II.            Demonstration of malarial parasite in peripheral smear

Rapid test for malaria and QBC

1. Demonstration of embryonatedegginoculation Demonstration of cell culture techniques and Cytopathic effect

Demonstration of heamagglutination and heamagglutination inhibition assay