BMLT | MICROBIOLOGY | First Year

 MICROBIOLOGY 1st Year

For Syllabus (Microbiology paper 1)

Sl. No Topics Domain Time

(Total-70 hrs)

1 General Microbiology

Microscopy

Light microscope,

Dark field,

Phase contrast microscopy,

Fluorescent & Electron microscopy

 

Must know

Desirable to know

Nice to know

Must know

1

1

1

1

 

2 Sterilization and Disinfection

Physical Methods of Sterilization

Chemical Methods of Sterilization

Methods of Disinfection

 

Must know

 

2

3 Growth,

Nutrition &

 Metabolism of Bacteria  

Must know

Desirable to know

Nice to know

1

1

1

4 Bacterial genetics  Must know 5
5 Bacterial Culture and Identification

(a) Culture Media & Transport Media

(b)Sample collection and transport Methods

(c)Aerobic Bacterial Culture Techniques

(d)Anaerobic Bacterial Culture Techniques

(e)Smear preparation & Staining methods

(f)Principle and techniques of biochemical Test

 Must know

Must know

Must know

Nice to know

Must know

Must know

1

1

3

1

1

 

1

6 Morphology,

Classification

& infection caused by Microorganisms (in brief)

Bacteria

Morphology, Classification

Human infection caused by bacteria

Must know

Nice to know

 

 

 

Must to know

1

1

 

 

 

 

2

7 Viruses

Morphology,

Classification

Human infection caused by Viruses

Must know

Nice to know

Must know

1

1

2

8 Fungi 

Morphology,

Classification

Human infection caused by Fungi

Must know

Nice to know

Must know

 

1

1

2

9 Parasites               

Morphology,

Classification

Human infection caused by Parasite

 

Must know

Nice to know

Must know

 

1

1

2

10 Immunology

Immunity

Antigen & Antibody

Antigen antibody reactions I

Precipitation, Agglutination

Antigen antibody reactions II

Complement fixation, Neutralization, ELISA, RIA, IF

Must know

Must know

Desirable to know

Nice to know

 

2

5

5

3

 

11 Antimicrobial Sensitivity Testing

-Antimicrobials and their mode of action

-Mechanisms of drug resistance                                                                          

-Antimicrobial susceptibility testing

(a)Diffusion Methods

(b)Dilution Methods

Must know

Must know

Desirable to know

Must know

1

2

1

 

 

 

3

12 Systemic Bacteriology

  • Morphology, culture characteristic, identification, diseases caused and laboratory diagnosis of following bacteria.
  • Staphylococcus,
  • Streptococcus,
  • Streptococcus pneumonae
  • Neisseria,
  • Corynebacterium,
  • Clostridium,
  • Mycobacteria,
  • Bacillus,
  • Shigella,
  • Salmonella,
  • E.coli,
  • Klebsiella, Proteus,
  • Vibrio, Pseudomonas
  • Spirochetes
 

Must know

 

 

 

Desirable to know

 

 

Must know

 

 

Nice to know

 

2

 

2

 

5

 

 

 

1

 

Syllabus (Microbiology practical paper 1)

S. No Topic Domain Hours

Total-30

1. 1.      Microscope

a.       Light Microscope

2.      Staining

a.       Gramsstaining

b.      ZN staining

3.      Preparation of commonly used culture media

a.       Nutrient Agar

b.      Blood Agar

c.       Chocolate agar

d.      Mac Conkey agar

e.       Muller Hinton agar

4.      Culturemethods

a.       Streak method

b.      Lawn method

c.       Stroke method

d.      Stab method

e.       Pour Plate method

f.        Liquid method

5.      Identificationofbacterialculture

a.       Colonycharacteristics

b.      Morphological characteristics

6.      Antibiotic susceptibility test

a.       Diffusion methods

b.      Dilution Methods

7.      Bio medical waste

a.       Use of colour coded bags

b.      Black

c.       Blue

d.      Red

e.       Yellow

8.      Demonstration of Sterilization & Disinfection method

a.       Autoclave

b.      Hot Air oven

c.       Chemical Sterilization

9.      Collection of specimen

a.       From outpatient units

b.      Inpatient units

c.       Minor operation theatre

d.      Major operation theatre for sterility testing

10.  Demonstration of personal protective equipment

 

Must know

 

Must know

 

Must know

 

 

 

 

Must know

 

Must know

 

 

Must know

 

Must know

 

 

Must know

 

 

Must know

 

 

 

Must know

2

 

2

 

2

 

 

 

 

2

 

2

 

 

2

 

2

 

 

2

 

 

2

 

 

 

2

2. 1.      Disinfection of wards, OT and Laboratory

2.      Visit to CSSD

Desirable to know 5
3. Demonstration of Sterilization & Disinfection method

a.       Water bath

b.      Inspissator

Sterility testing

o   Methods

o   Interpretation

 

Nice to know 5

 

 

 

 

 

 

MICROBIOLOGY REFERENCES :

Laboratory acquired infections by C.H. Collins

Manual of Clinical Laboratory methods by Copal E Hopier

Medical Laboratory Methods By Beuer

Bailey & Scott’s diagnostic microbiology (13th ed.) by Tille, P. M.

Elsevier’s medical laboratory science examination review by Graeter, L. J., Hertenstein, E. G., Accurso, C. E., &Labiner, G. H.

Fundamentals of urine and body fluid analysis (3rd ed.) by Brunzel, N. A.

Immunology and serology in laboratory medicine (5th ed.) by Turgeon, M. L.

SGTUNIVERSITY
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